Paradoxical inhibition of cardiac lipid peroxidation in. Lipid peroxidation was calculated by relating the emission peak at 520 nm to the sum of the fluorescence emission peaks fo at 520 and. That cytochrome p450 would act in its oxidized form appears unlikely in view of the enhanced nadph oxidation that ac companies lipid peroxidation. Lipid peroxidation in isolated membranes of cerebral. Malondialdehyde was not detected in the actual vessel wall of the mca of any group. Fish were exposed to sublethal concentrations of butachlor 1, 2, 2. An analysis of merits and demerits t p a devasagayam 1, k k boloor 1 and t ramasarma 2 1radiation biology and health sciences division, bhabha atomic research centre, mumbai 400 085 2insa honorary scientist, solid state and structural chemistry unit and department of biochemistry. Thiobarbituric acid reactive substances tbars were measured as a marker of lipid peroxidation in plasma by fluorometric determination and tissues by colorimetric determination buege and aust 1978. Purpose to study the lipid hydroperoxide activity in vasoproliferative and fibroproliferative retinal disorders. Peroxidation of membrane lipids by this system is equally effective in both intact microsomes and in liposomes, indicating that iodination of microsomal protein is not required for lipid peroxidation to occur. Moreover, soy supplementation improved the antioxidant enzymes, decreased lipid peroxidation, and improved the histological picture of the liver tissue.
Lipid peroxidation is a complex process known to occur in both plants and animals. Detection ultraviolet lightinduced increase, pathogenic importance. Ascorbic acid ah 2 is a potential scavenger of superoxide radical and singlet oxygen. Determination of serum aluminum, platelet aggregation and. Lipid peroxidation in proliferative vitreoretinopathies eye. Lipid peroxidation was evaluated through the quantification of species that are reactive to thiobarbituric acid tba, as described by buege and aust 1978.
Cyp2e1, the main enzyme associated with that process in wildtype mice, is. Rat liver microsomal lipid peroxidation produced during the. The effects of aged garlic extract age on lipid peroxidative damage and the deformability of erythrocytes were evaluated in rats. The microsomal lipid peroxidation system of flounder muscle was characterized as to response to temperature, cofactor requirements, inhibitors, and activators. In the guinea pig, marginal ah 2 deficiency results in intracellular oxidative damage in the cardiac tissue as evidenced by lipid peroxidation, formation of fluorescent pigment and loss of structural integrity of the microsomal membranes. Lipid peroxidation of liver and kidney microsomes induces a highly characteristic sequence of morphological changes typified by detachment of ribosomes and formation of large aggregates of vesicles bound together by dense amorphous material and myelin figurelike debris. Fatty acid analysis of the microsomal lipid from passaged tumours demonstrated adequate levels of substrate in the phospholipid fractions to support lipid peroxidation. Lipid peroxidation by ultraviolet light and high energy. A significant increase in malondialdehyde formation was observed in the liver. A possible mechanism for initiation of lipid peroxidation. In this context, the induction of antioxidant systems in fish collected from polluted area in response to oxidative stress should be considered as a clear indication of the presence of pollution and environmental health degradation. The test results on lipid oxidation indicated that after 15 days of refrigerated storage at 4c, oxidation in the control group was 2. The role of nadphcytochrome p 450 reductase in lipid peroxidation was established, in part, by the ability of antibody to the reductase to inhibit nadph dependent microsomal lipid peroxidation 10. Pdf on jan 1, 1978, john a buege and others published microsomal lipid peroxidation find, read and cite all the research you need on researchgate.
It involves the formation and propagation of lipid radicals, the uptake of oxygen, a rearrangement of the double bonds in unsaturated lipids, and the eventual destruction of membrane lipids, producing a variety of breakdown products, including alcohols. Lipid peroxidation method general biology discussion. The enzymatic reaction required adp, nadph or nadh, and ferrous or ferric iron. The trilaminar structure of the membrane is, however, retained even after complete peroxidation, though. Estimation of other products of lipid peroxidation.
Omega3 improves glucose tolerance but increases lipid peroxidation and dna damage in hepatocytes of fructosefed rats. Centrifuge the homogenate for 10 min 15000 x g, 4,0 oc 3. Aust, microsomal lipid peroxidation, methods in enzymology, vol. Cooxidation of the amine substrate occurs during ironascorbatepromoted lipid peroxidation also, but h2o2 or free oh. Kinetic activity, membrane mitochondrial potential, lipid. Endrin toxicity may be due to an oxidative stress associated with increased lipid peroxidation, decreased glutathione content, and inhibition of glutathione peroxidase activity. Just as in the case of mixedfunction oxidation, cytochrome p450, in conjunction with nadphcytochrome p450 re ductase. Fleischer s, packer l eds methods in enzymology, vol 52c.
Salivary lipid peroxidation and lipid profile levels in patients with recent ischemic stroke article pdf available in journal of international dental and medical research 32. The deformability of erythrocytes was measured using the micropore filtration method. Collect supernatant and mix 0,5 ml of supernatant with 1,5 ml 0,5%. The two fish species accumulated the heavy metals pb and zn in their liver, muscle and gill with the highest concentration of the heavy metals in. The influence of exercise combined with wholebody cryotherapy wbc on the oxidantantioxidant balance in healthy men was assessed.
Methods enzymol 52, 302310 this is not available online anywhere and i need this paper. Post juveniles of clarias gariepinus and oreochromis niloticus were exposed to 10% 96hrlc 50 values of pb no 3 2 and zncl 2 against each species respectively, for 30 days. Some characteristics of the enzymic lipid peroxidation. These results suggest that iron may be a causal agent of cisplatininduced lipid peroxidation. The function of this additional component in the lipid peroxidation. Furthermore, hepatic microsomal lipid peroxidation was substantially inhibited by antimouse cyp4a10 antibody in vitro. Methods vitreous body samples from patients undergoing vitrectomy because of. Microsomal lipid peroxidation 151 figure 6 summarizes in a schematic form our current and tentative view on the possible role of cytochrome p450 in microsomal lipid peroxidation. Therefore, histopathological changes and lipid peroxidation in the livers and kidneys of rats, mice, hamsters, and guinea pigs were examined 24. Pdf salivary lipid peroxidation and lipid profile levels.
The oxidative damage does not occur due to lack of enzymatic. The binding of positively charged species to a membrane to the negativelycharged head. Homogenize 0,1 gr of leaf tissue by adding 0,5 ml 0,1 % wv tca 2. Diezel w, sonnichsen n 1976 stable lipid peroxidation products in human skin. The role of reduced nicotinamide adenine dinucleotide phosphatecytochrome c reductase in liver. Effect of fatty acid saturation on nadphdependent lipid. These results show that experimental nash is strongly associated with hepatic microsomal lipid peroxidation. Positive effects of green tea on hepatic dysfunction. Toxicity from lipid peroxidation affect the liver lipid metabolism where cytochrome p450s is an efficient catalyst in the oxidative transformation of lipid derived aldehydes to carboxylic acids adding a new facet to the biological activity of lipid oxidation.
However, superoxide dismutase has iittle antioxidant effect on the lipid peroxidation of microsames which ca talyze the production of superoxide an. The study included 16 kayakers of the polish national team, aged 22. Effects of aged garlic extract on lipid peroxidation and. Buege ja, aust sd 1978 microsomal lipid peroxidation. Mda formation was quantitated using a molar extinction coeficient of 1. No statistic correlation between superoxide dismutase and peroxidase activities and aluminuminduced lipid peroxidation in maize, implying limited roles of both enzymes in prevention against aluminuminduced lipid peroxidation. Extensive interspecies variability exists in sensitivity towards endrin. Accumulation and antioxidant enzymes as biomarkers of heavy metal exposure in clarias gariepinus and oreochromis niloticus. It involves the formation and propagation of lipid radicals, the uptake of oxygen, a rearrangement of the double bonds in unsaturated lipids. Aust lipid peroxidation is a complex process known to occur in both plants and animals. Age significantly prevented the decrease of erythrocyte deformability induced by lipid peroxidation in a dosedependent manner. Aust u lipid peroxidation is a complex process known to occur in both plants and animals. Lactoperoxidasecatalyzed lipid peroxidation of microsomal. The mechanism of nadphdependent lipid peroxidation the propagation of lipid peroxidation received for publication, december 1, 1978 bruce a.
Buege ja, aust sd 1984 microsomal lipid peroxidation. The role of lipid peroxidation in the noxidation of 4. Free fatty acids and their oxidation products can be measured by hplc 51. The requirement for iron iii in the initiation of lipid peroxidation by iron ii and hydrogen peroxide. It could be concluded that soyproteinenriched isoflavones may be a.
Also, the increase in lipid peroxidation was a useful indicator of the pollution load in the present study. Effect of butachlor on antioxidant enzyme status and lipid. The present study was undertaken to evaluate the influence of butachlor, a widely used herbicide, on antioxidant enzyme system and lipid peroxidation formation in african cat fish clarias gariepinus. This chapter discusses microsomal lipid peroxidation. Thus, 02 may not be involved in microsomal lipid peroxidation or su. Effect of 21aminosteroid u74006f on lipid peroxidation. Aluminum, platelet aggregation and lipid peroxidation in hemodialysis brazilian journal of medical and biological research 2002 35. Microsomal electron transport journal of biological. Liang wang, jianyu zhao, sanmin wu, jianglong pan, zhangbao huang, zikai wu, xianwei fan, youzhi li. Indeed, noxidation of 4chloroaniline is fully preserved when either phospholipid liposomes or dispersed linoleic acid substitute for intact microsomal fraction. Svingen and aust 6, 54 suggested a mechanism for lipid peroxidation in microsomes that. The aim of this study was to examine concen trations and activities of microsomal membrane bound enzymes during different levels of microsomal lipid peroxidation in vitro, so as to relate any changes in these enzyme activities to morphological and chemical alterations of the membrane. Lactoperoxidasecatalyzed lipid peroxidation of microsomal and artificial membranes.
Studies of lipid peroxidation in microsomes isolated from. Read increased lipid peroxidation and antioxidant activity in methionineinduced hepatitis in rabbits, nutrition on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. These results suggest that lipid peroxidation in subarachnoid clot may play a role in the pathogenesis of vasospasm and that the salutary effects of u74006f in vasospasm may be mediated by a reduction of lipid peroxidation in sah. Effect of prolonged intake of iron enriched diet on testicular functions of experimental rats authors. The 400 ppm and 800 ppm of catechintreated groups yielded 0. Two classical inhibitors of cytochrome p450dependent microsomal hydroxylation reactions, metyrapone and skf 525a, inhibit the nadphdependent lipid peroxidation. The iron chelator deferoxamine prevents cisplatin induced. Two different methods were applied for determining tbars in plasma and in tissues to avoid interference by iron ions and to use a sufficiently. Malondialdehyde formation is not dependent upon the production of superoxide, singlet oxygen, or hydroxyl radicals. Omega3 improves glucose tolerance but increases lipid. Components of the ain93 diets as improvements deficient diet had lymphocyte dna damage, as assessed by in the ain76a diet. Mechanisms and consequences of lipid peroxidation in biological. The presence of an enzymatic lipid peroxidation system in microsomal fractions of beef or pork muscle has been demonstrated in this study.
660 595 29 773 65 292 1148 1310 885 1406 133 44 551 54 1153 1464 1074 1388 727 321 1057 1351 757 23 749 986 1004 1070 849 827